EP4 Receptor

Prostanoids are the cyclooxygenase metabolites derived from C-20 unsaturated fatty acids (arachidonic acid) and include prostaglandin (PG) D2, PGE2, PGF2 alpha, PGI2, and thrombaxane (Tx) A2. The fatty acids precursors are released from the membrane phosphoplipids in response to various physiological and pathological stimuli by the action of phospholipase A2 and are converted to various prostanoids by the sequential actions of cyclooxygenases and the respective synthases. Prostaglandin PGE2 is one of the major prostaglandin produced during inflammation. A variety of PGE2-mediated effects on vascular smooth muscle tonus, glomerular cell function, renin release, and renal salt and water transport have been described. PGs also influence neuronal activity by modulating neurotransmitter release, sensitizing secretory fibers to noxious stimuli, or inducing fever and sleep. The actions of PGE2 are mediated by rhodopsin-type; G-protein coupled membrane receptors, termed E-prostanoid (EP) receptors or PTGERs. There are four subtypes of PGE receptors designated as EP1, EP2, EP3, and EP4 that are encoded by different genes and expressed differently in each tissue. The intracellular signaling also differs among the receptor subtypes.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.5-1ug/ml. Control peptide can be used to coat plate at 1ug/ml\nWestern Blot: 1-10ug/ml (ECL). EP4 has been shown to be 47 and 78kD EP4 bands in the kidney.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: \nE3364-48: EP4 Receptor, Human, Control Peptide\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3364-46

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Bastien L et al (1994) J. Biol. Chem. 269, 11873. 2. An S et al (1993) BBRC 197, 263. 3. Foord SM et al (1996) Genomics 35, 182. 4. Mori K et al (199J. Mol. Med. 74, 333-336. 5. Narumiya S et al (1999) Physiol. Rev. 79, 1193. 6. Morath R et al (1999) J Am. Soc. Nephrol. 10, 1860;

Additional Info

Recognizes human EP4 Receptor. No significant homology with other EP (1, 2 or 3) receptors.