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$EpCAM (CD326, Epithelial Cell Adhesion Molecule, \nEpithelial Cell Surface Antigen)$

Supplier:

United States Biological

Cat no: 138542

EpCAM (CD326, Epithelial Cell Adhesion Molecule, \nEpithelial Cell Surface Antigen)

EpCAM, or CD326, is an ~40kD transmembrane glycoprotein involved in a number of cellular processes including adhesion, proliferation, maintenance of stemness, migration and invasion (Spizzo, 2012, Denzel, 2012). It is expressed on most, but not all, normal epithelia and their corresponding malignancies. Although the role of EpCAM expression in tumorigenesis remains to be fully elucidated, it may downregulate immunity and help tumors actively escape from immune surveillance. Since the 1980s, EpCAM has been recognized as a pan-carcinoma antigen marker because of its widespread expression on epithelium and their derived tumors (Litnov, 1994; Ruf, 2007). More recently, it has also been identified as a stem cell marker, and it is thought that EpCAM antibody positive tumor cells may have stem-like properties (Flatmark, 2011). EpCAM antibody is widely used to help distinguish epithelial from non-epithelial neoplasms. EpCAM antibody positive tumors are epithelium derived, whereas EpCAM antibody negative tumors can originate from either non-epithelial or epithelial tissues. EpCAM antibody has also been used for identifying circulating tumor cells (Saif, 2012, de Albuquerque, 2012). It is often used as part of a panel with other tumor or tissue antibody markers when classifying tumors of known, suspected or elusive origin. EpCAM expression may be upregulated in tumors and overexpression can most pronounced on tumor initiating cells (Imrich, 2012). Overexpression may be an indication of early malignancy and correlate with a poor prognosis (Ruf, 2007).\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:50-1:100\nImmunohistochemistry(Paraffin): 1:200-1:400\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

138542

Size

100ul

Applications

IHC, WB

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in 0.05% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. de Alburquerque A, S Kaul, G Breier, P Krabisch, N Fersis. Breast Care 7:7-12 (2012). 2. Ruf P, O Gires, M Jager, K Fellinger, J Atz, H Lindhofer. British J Cancer 97:316-321 (2007). 3. Saif MW. JOP (http://www.serena.unina.it/index.php/job). 13:124-127 (2012). Antibody techniques for detecting circulating pancreatic tumor cells. 4. Denzel S, B Mack, C Eggert, P Massoner, N Stocklein, D Kemming, U Harreus, O Gires. Int J Exp Path 93:341-353 (2012) 5. Imrich S, M Hachmeister, O Gires. Cell Adhes Migr 6:30-38 (2012). 6. Spizzo G, D Fong, M Wurm, C Ensinger, P Obrist, C Hofer, G Mazzoleni, G Gastl, P Went. J Clin Pathol 64:415-420 (2011). 7. Litvinov SV, MP Velders, HAM Bakker, GJ Fleuren, SO Warnaar. JBC 125:437-446 (1994).

Additional Info

Recognizes rat EpCAM.