EphA3 Receptor (Ephrin A3 Receptor)

Eph receptors belong to a family of receptor tyrosine kinases. Both ephrins, the ligands for Eph receptors, and Eph receptors are broadly expressed throughout the ectoderm, mesoderm, and endoderm of vertebrate embryos. The interaction of ephrins with the appropriate Eph receptor(s) has been implicated in a wide spectrum of functions in vertebrate morphogenesis, including the accurate migration of embryonic cells, formation of boundaries between structures (i.e. rhombomeres and somites), and the control of cytoskeletal changes which dictate cellular shape and adhesion during development. EphA3 receptor, also known as Cek4, Mek4, Hek, Tyro4 and Hek4, is thought to repel receptor positive myoblasts and axons away from the limb bud and towards the body wall. EphA3 receptor is involved in targeting of the retinotectal projection and the organization of the retinotectal pathway.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications have not been tested.\n\nRecommended Dilutions:\nELISA (Immunogen): 0.1-1ug/ml\nWestern Blot (Rat): 1-3ug/ml, detects a band at ~110kD.\nImmunoprecipitation (Rat): 5-10ug\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman EphA3 receptor transfected HEK-293 cell lysate.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3365-03

Size

100ug

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography

Isotype

IgG2a,k

References

1. Holder, N. and Klein, R., Development 126(10): 2033-2044 (1999). 2. Flanagan, J.G. and Vanderhaeghen, P., Annu. Rev. Neurosci. 21: 309-345 (1998). 3. Pasquale, E.B., Curr. Opin. Cell Biol. 9(5): 608-615 (1997).

Additional Info

Recognizes human EphA3 Receptor. On Western Blot, this antibody reacts specifically with recombinant human EphA3 receptor without crossreaction with EphA4 receptor expressed in transfected HEK-293 cells. Species Sequence Homology: mouse and rat.