EphA4 Receptor (Ephrin A4 Receptor)

The Eph receptors are the largest family of receptor tyrosine kinases. They can be grouped into two subclasses based on sequence homology: the EphA and the EphB receptors. The ligands for the Eph receptors are the ephrins. The EphA receptors bind ephrin-A ligands, which are tethered to the membrane by a GPI linkage, and the EphB receptors bind ephrin-B ligands, which have a membrane spanning region. The Eph receptors are widely expressed during embryonic\ndevelopment, including in the nervous system and the vascular system. Ephrins and Eph receptors have been implicated in many developmental processes including neuronal network formation, guidance of cell migration, and axonal pathfinding. They remain expressed at lower levels in adult tissues and are upregulated in pathological conditions such as cancer.\nEphA4 is preferentially expressed in the embryonic and adult nervous system, including the adult hippocampus, and in platelets. EphA4 has previously been designated Cek8 in chicken, Tyro1 in rat, and Sek1 in mouse.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot (Human, mouse, chicken): 1-3ug/ml\nImmunoprecipitation (Human, mouse, chicken): 5ug\nImmunohistochemistry (Frozen, mouse): 3-10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nWestern Blot, Immunoprecipitation: EphA4-transfected 293T cells and mouse brain lysates.\nImmunohistochemistry: EphA4-transfected 293T cells and mouse hippocampus.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3362-01D

Size

100ug

Applications

ELISA, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1,k

References

1. Holder, N., et al., Development 126(10): 2033-2044 (1999). 2. Flanagan, J.D., et al., Annu. Rev. Neurosci. 21: 309-345 (1998). 3. Pasquale, E.B., Curr. Opin. Cell Biol. 9(5): 608-615 (1997). 4. Murai, K.K., et al., Nature Neurosci. 6(2): 153-160 (2003). 5. Eph Nomenclature Committee, Cell 90(3): 403-404 (1997).

Additional Info

Recognizes the endogenous human EphA4 receptor protein at ~110-120kD. No crossreactivity with EphA5 or EphA7 has been detected.