EphB1 (Ephrin Type-B Receptor 1, Tyrosine-protein Kinase Receptor EPH-2, NET, EPH-like Kinase 6, EK6, hEK6, HEK-6, EPHT2)

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3371-01L

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Pab

Purity

Purified by ammonium sulfate precipitation.

Isotype

IgG

References

1.Prevost, N., et al., Proc. Natl. Acad. Sci. USA 99(14):9219-9224 (2002). 2.Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001). 3.Xu, Q., et al., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 355(1399):993-1002 (2000). 4.Holder, N., et al., Development 126(10):2033-2044 (1999). 5.Stein, E., et al., J. Biol. Chem. 273(3):1303-1308 (1998).

Additional Info

Recognizes human EphB1.