Ephrin A1 (ECKLG, EFL1, EPH-related receptor tyrosine kinase ligand 1, Ephrin-A1, EPLG1)

Ephrins are the ligands which bind to the Eph receptor subfamily, the largest group of the receptor tyrosine kinase family. Both ephrins and Eph receptors are broadly expressed throughout the ectoderm, mesoderm, and endoderm of vertebrate embryos.1 The interaction of ephrins with the appropriate Eph receptor(s) has been implicated in a wide spectrum of functions in vertebrate morphogenesis, including the accurate migration of embryonic cells,2 formation of boundaries between structures (i.e. rhombomeres and somites), and the control of cytoskeletal changes which dictate cellular shape and adhesion during development. Two classes of ephrins exist: Class A ephrins are tethered to the membrane by a GPI linkage and bind primarily to EphA receptors; Class B ephrins contain a membrane-spanning region and bind predominantly to EphB receptors. Ephrin-A1, also known as B61, LERK-1, and EFL1,3 is a Class A protein. It has been shown to bind the EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, and EphB2 receptors.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-5ug/ml\nImmunoprecipitation: 5-10ug/IP reaction \nImmunohistochemistry: 4-10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3355

Size

100ug

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes the mouse ephrin-A1 protein. Reactivity is con?rmed with a chimeric protein consisting of the extracellular domain of mouse ephrin-A1 and the Fc region of human IgG1. Crossreactivity with human ephrin-A1 is con?rmed with Immunohistochemistry experiments on human tissue sections and this reactivity was expected because of the 85% shared amino acid identity in the extracellular domain.