Ephrin-A4 (the antigen to this antibody), also known as LERK-4 or ELF-4. Ephrin-A4 binds EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1. Presently, 14 members of the Eph receptor family and a family of 8 ligands have been identified. Ligands of Eph family receptors are structurally related membrane-bound proteins that can be subdivided into two major subclasses: ephrin-A and ephrin-B. Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl- inositollinkages; whereas ephrin-B subclass consists of ligands with transmembrane domains.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:100 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive Control:
Brain
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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