Ephrin A4 Recombinant, Human (EPH-related receptor tyrosine kinase ligand 4)

Ephrins are the ligands which bind to the Eph receptor subfamily, the largest group of the receptor tyrosine kinase family. Both ephrins and Eph receptors are broadly expressed through the ectoderm,mesoderm, and endoderm of vertebrate embryos. Two classes of ephrins exist: Class A ephrins are tethered to the membrane by a GPI linkage and bind primarily to EphA receptors; Class B ephrins contain a membrane-spanning region and bind predominantly to EphB receptors.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Inhibition assays. Other applications have not been tested. \n\nRecommended Dilutions: \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3355-05

Size

2ug

Form

Supplied as a liquid in 50mM Tris-acetate, pH 7.5, 1mM EDTA, 20% glycerol.

Purity

Purified by proprietary chromatographic techniques. Shows a 46kD SDS-PAGE (Including GST Tag).

References

1.Ephrin-A4 inhibits sensory neurite outgrowth and is regulated by neonatal skin wounding. Eur J Neurosci 2005 Nov;22(10):2413-21. 2. Characterization of the human ephrin-A4 promoter. Biochem J 2002 Sep 1;366(Pt 2):447-58. 3. A splice variant of human ephrin-A4 encodes a soluble molecule that is secreted by activated human B lymphocytes. Blood 2000 Jan 1;95(1):221-30. 4. Characterization of ephrin-A1 and ephrin-A4 as ligands for the EphA8 receptor protein tyrosine kinase. Mol Cells 1999 Aug 31;9(4):440-5.