Ephrin-A5 (ephrin-A5, AF1, AL-1, EFL5, EPH- related receptor tyrosine kinase ligand 7, Ephrin-A5 precursor, EPLG7, GLC1M, LERK7, LERK-7, RAGS)

Ephrins are ligands, that bind to the Eph receptors, the largest group of the receptor tyrosine kinase subgroup. Both ephrins and Eph receptors are broadly expressed throughout the ectoderm, mesoderm, and endoderm of vertebrate embryos.1 The interaction of ephrins with the appropriate Eph receptor(s) has been implicated in a wide spectrum of functions in vertebrate morphogenesis, including the accurate migration of embryonic cells,2 formation of boundaries between structures (i.e. rhombomeres and somites), and the control of cytoskeletal\nchanges which dictate cellular shape and adhesion during development. The ephrin-A5 modulates cell adhesion and morphology by specifying the affinity of cells towards various extracellular substrates by regulating integrin function.3 It also selectively inhibits spinal cord neurite outgrowth and cell survival, thus regulating morphogenesis and axon guidance in the spinal cord.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot, Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 5ug/ml\nWestern Blot: 1-3ug/ml\nImmunoprecipitation: 5-10ug/ IP reaction\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

E3363-01C

Size

50ug

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. before the addition of glycerol to 40%

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Holder N, Klein R., (1999) Development 126(10):2033-2044. 2. Flanagan JG, (1998) Vandehaeghen P. Annu Rev Neurosci 21:309-345. 3. Davy A, et al., (2000) Embo J 19:5396-5405. 4. Wahl S, et al., (2000) J Cell Biol 149:263-270.

Additional Info

Recognizes the mouse ~25kD ephrin-A5 protein. Reactivity is confirmed with ephrin-A5 transfected 293 cell lysates in both western blot and immunofluorescence assays. Species sequence homology: human and rat