Ephrin B, pan

Ephrins are a family of proteins that bind to the tyrosine kinase Eph receptor. Their function is still under investigation, but they are suspected to play an important role in morphogenesis. The ephrins can be grouped into two classes: those that are tethered to the membrane by a GPI linkage, called ephrin-A proteins (which bind to EphA receptors), and those with a membrane-spanning region, called ephrin-B proteins (which bind to EphB receptors). Ephrins are expressed in the embryo in various tissues in the forming mesoderm, endoderm and nervous system in all of the vertebrate species in which they have been identified. Ephrin-B3, also known as NLERK-2, Elk-L3, EFL-6, ELF-3 and LERK-8 is a member of the ephrin-B protein family. Ephrin-B3 binds EphA4, EphB1, EphB2 and EphB33,. Ephrin-B1 is one of the transmembrane ligands for the EphB2 receptor. In the embryo, EphB2 and ephrin-B1 participate in neuronal axon guidance, neural crest cell migration, the formation of blood vessels and the development of facial structures and the inner ear. EphB2 and ephrin-B1 can both signal through their cytoplasmic domains and become phosphorylated when bound to each other. Tyrosine phosphorylation regulates EphB2 signaling and likely also ephrin-B1 signaling.\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 1-3ug/ml\nImmunoprecipitation: 5ug/ml\nELISA: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Controls:\nHuman 293T and MCF10A lysates, chicken cell lysates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3371-01B

Size

50ug

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum, Ch/Bird

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide

P Type

Mab

Purity

Purified by Protein A chromatography

Isotype

IgG1,k

References

1. Holder N and Klein R. Development 126(10): 2033-2044, 1999. 2. Ephrin Nomenclature Committee. Cell 90(3): 403-404, 1997. 3. Flanagan JG, and Vanderhagen P. Annu Rev Neurosci 21: 309-345, 1998. 4. Pasquale EB. Curr opin Cell Biol 9(5): 608-615, 1997. 5. Kalo MS et al. J Biol Chem 276(42): 38940-38948, 2001.

Additional Info

Recognizes over-expressed and endogenous ephrin-B, as well as phosphorylated ephrin-B. On Western Blots it identifies a band at ~50-60kD. Species crossreactivity: human and chicken. Species sequence homology: mouse