Ephrin B, phosphorylated (Tyr331)

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event. Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling. (3, 5)\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3371-01E

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Ch/Bird

Form

10 mM Hepes (pH7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Bong, Y.S., et al., (2004) Biochem. J. 377:499-507. 2. Dufour, A., et al., (2003) Neuron 39:453-465. 3. Flanagan, J.G. and Vanderhaeghen, P. (1998) Annu. Rev. Neurosci. 21:309-345. 4. Oike, Y., et al., Blood (2002) 100:1326-1333. 5. Palmer, A., et al., (2002) Mol Cell 9:725-737.

Additional Info

Specific for the ~46k EphrinB protein phosphorylated at Tyr331. The immunolabeling of the EphrinB band is blocked by treatment with lambda-phosphatase. Species Crossreactivity: Chicken, human, mouse, rat, and Xenopus based on 100% antigen sequence homology.