Ephrin B1, Recombinant, Human

Ephrins are the ligands which bind to the Eph receptor subfamily, the largest group of the receptor tyrosine kinase family. Both ephrins and Eph receptors are broadly expressed through the ectoderm, mesoderm, and endoderm of vertebrate embryos. Two classes of ephrins exist: Class A ephrins are tethered to the membrane by a GPI linkage and bind primarily to EphA receptors; Class B ephrins contain a membrane-spanning region and bind predominantly to EphB receptors.\n\nEFNB1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 231 amino acids (28-237) and having a molecular mass of 25.3kD.\nThe EFNB1 is fused to a 21aa His-Tag at the N-terminus \n\nApplications:\nSuitable for use in ELISA, Inhibition Assays and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nAmino Acid Sequence:\nMGSSHHHHHH SSGLVPRGSH MLAKNLEPVS WSSLNPKFLS GKGLVIYPKI GDKLDIICPR AEAGRPYEYY KLYLVRPEQA AACSTVLDPN VLVTCNRPEQ EIRFTIKFQE FSPNYMGLEF KKHHDYYITS TSNGSLEGLE NREGGVCRTR TMKIIMKVGQ DPNAVTPEQL TTSRPSKEAD NTVKMATQAP GSRGSLGDSD GKHETVNQEE KSGPGASGGS SGDPDGFFNS K\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3371-01H

Size

5ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.4M urea, 5% glycerol.

Purity

~95% by SDS-PAGE

References

1. Distribution of EphB receptors and ephrin-B1 in the developing vertebrate spinal cord. J Comp Neurol 2006 Aug 10;497(5):734-50. 2. Ephrin-B1 is critical in T-cell development. J Biol Chem 2006 Apr 14;281(15):10222-9. 3. EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases. J Cell Sci 2006 Feb 1;119(Pt 3):559-70. 4. Phosphorylation of ephrin-B1 via the interaction with claudin following cell-cell contact formation. EMBO J 2005 Nov 2;24(21):3700-11. 5. Crystal structure of the ephrin-B1 ectodomain: implications for receptor recognition and signaling. Biochemistry 2005 Aug 23;44(33):10947-53. 6. Induction of ephrin-B1 and EphB receptors during denervation-induced plasticity in the adult mouse hippocampus. Eur J Neurosci 2005 May;21(9):2336-46.