Epiandrosterone is a steroid hormone with weak androgenic activity. It is a natural metabolite of dehydroepiandrosterone via the 5-alpha reductase enzyme. It was first isolated in 1931, by Adolf Friedrich Johann Butenandt and Kurt Tscherning. They distilled over 17000 litres of male urine, from which they got 50 milligrams of crystalline androsterone (most likely mixed isomers), which was sufficient to find that the chemical formula was very similar to estrone. Epiandrosterone has been shown to naturally occur in most mammals including pigs. Epiandrosterone is naturally produced by the enzyme 5-alpha reductase from the adrenal hormone dehydroepiandrosterone (DHEA). Epiandrosterone can also be converted from the natural steroids Androstanediol via 17b-Hydroxysteroid dehydrogenase or from Androstanedione via 3b-Hydroxysteroid dehydrogenase.
Synonyms: 3-Epiandrosterone, 3.beta.-Androsterone, 3.beta-Hydroxyetioallocholan-17-one, 5.alpha-Androstan-17-one, 3.beta-hydroxy-, 5.beta-Androstan-17-one, 3.alpha-hydroxy-, 5beta.-Androstan-17-one, 3-beta-Hydroxyetiollocholan-17-one
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
ELISA: 1.25ug/ml
Optimal dilutions to be determined by the researcher.
Match To: E3357-01
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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