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Epidermal Growth Factor (EGF, beta-Urogastrone, HOMG4, URG)

Epidermal Growth Factor (EGF, beta-Urogastrone, HOMG4, URG)

Cat no: E3374-06B

Supplier: United States Biological
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The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the 170kDa EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-a, VGF (vaccinia virus growth factor) and Betacellulin, produced by certain tumor cells. Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways. Applications: Suitable for use in ELISA, Functional Assays, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 0.25-1ug/ml ELISA: 0.5-2ug/ml Western Blot: 0.1-0.2ug/ml Functional Assays: 0.25-0.35ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human skin Storage and Stability: Lyophilized powder may be stored at -20 degrees C for short-term only.Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E3374-06B
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile 40-50% glycerol, ddH2O.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Carpenter, G. and Cohen, S. (1990) Epidermal Growth Factor*. J. Biol. Chem. 265: 7709-7712. 2. Saito, T. et al. (2004) Differential activation of Epidermal Growth Factor (EGF) Receptor downstream signaling pathways by Betacellulin and EGF. Endocrinology. 145: 4232-4243.
Additional info: Recognizes human EGF.

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