Applications:
Suitable for use in ELISA, Western Blotting, Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:50,000
Western Blotting: 1:200-1:1000
Immunohistochemistry 1:20-1:50
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.