EMR3 has been reported to be expressed in neutrophils, monocytes, and macrophages. ESTs have been isolated from liver/spleen and mixed libraries. Human epidermal growth factor (EGF) module-containing mucin-like hormone receptor 3 (EMR3) is a ~56kD member of the EGF-7 transmembrane (TM7) family of adhesion receptors. EMR3 is expressed at the cell surface as a heterodimer.
Applications:
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:50-1:200
Optimal dilutions to be determined by the researcher.
Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.