Epidermal Growth Factor Receptor Ab multiple clones (erbB, EGFR)

Supplier:

United States Biological

Cat no: E3375-50

Epidermal Growth Factor Receptor Ab multiple clones (erbB, EGFR)

EGFR is the prototype member of the type 1 receptor tyrosine kinases. EGFR is encoded by the cellular oncogene c-erbB-1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxyterminal domain. The carboxyterminal domain contains at least four tyrosine autophosphorylation sites. EGFR over-expression in tumors indicates poor prognosis. EGFR over-expression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and stomach, and in squamous cell carcinoma.\n\nHybridoma: Immunized BALB/c mouse splenocytes were fused with NSO or NS1 mouse myeloma cells.\n\nApplications: \nSuitable for use in Western Blotting, Immunoprecipitation, Immunostaining. Other applications not tested.\n\nRecommended Dilution:\nWestern Blotting: 0.5-1mg/ml\nOptimal dilutions to be determined by the researcher.\n\nRecommended Positive Control: A431 cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

E3375-50

Size

100ug

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide

P Type

Mab

Purity

Purified from ascites by Protein A and Protein G affinity chromatography.

Isotype

IgG1+IgG2a

References

1. Wiley, L.M., et al. (1995) BioEssays 17:839-846. 2. Rajkumar, T., et al. (1994) Breast Cancer Res. Treat. 29:3-9. 3. Henrik Daub, F., et al. (1996) Nature 379:557-560.

Additional Info

This mixture of two monoclonal antibodies binds to proteins with Mr=170kD and 145kD, corresponding to the intact and vIII variant of EGFR. The epitopes for these antibodies map to the extracellular and cytoplasmic domains of EGFR. Species Reactivity: Human. Does not crossreact with mouse or rat.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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