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Epidermal Growth Factor Receptor, CT (erbB, EGFR)

Cat no: E3375-35F

Epidermal Growth Factor Receptor, CT (erbB, EGFR)

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.\n\nApplications:\nSuitable for use in ELISA, Immunoprecipitation and Western Blot. Optimal dilutions to be determined by researcher. \n\nRecommended Dilution:\nELISA: 0.05ug/ml\nWestern Blot: 1ug/ml for AP (BCAP, NBI) (DTT), 0.1ug/ml for (ECL)\nImmunoprecipitation: 1-10ug/10e6 vanadate treated A431 cells.\nOptimal dilutions to be determined by the researcher.\n\nIncluded Positive Control: \nH2030-03H, HepG2 Cell Lysate, Untreated\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3375-35F

Size

50ug

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide, PEG and sucrose.

P Type

Mab

Purity

Purified by thiophilic adsorption followed by size exclusion chromatography.

Isotype

IgG1,k

Additional Info

Recognizes EGFR (aa 1165-1186). Species crossreactivity: human and mouse.

Read more on Supplier website

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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