EGFR is over expressed in a high proportion of breast cancer cells and in a range of other carcinomas, high level expression of EGFR is often associated with advanced disease and poor prognosis.
Mutations to EGFR have been found in many human carcinomas including breast, brain, lung, and colon. Over-expression of more than one member of the ErbB family was found to have a negative synergistic effect on patient outcome.
Applications:
Suitable for use in Immunohistochemistry (paraffin). Other applications not tested.
Recommended Dilution:
Immunohistochemistry (paraffin): 2-5ug/ml
Requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.
Positive Control: Ovarian or breast carcinoma
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Quick Search
Products 
