Epidermal Growth Factor Receptor (erbB, EGFR) (APC)

The epidermal growth factor receptor (EGF R) subfamily of receptor tyrosine kinases comprises four members: EGF R (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins that have an extracellular domain containing two cysteine-rich domains separated by a spacer region that is involved in ligand-binding, and a cytoplasmic domain that has a membrane-proximal tyrosine kinase domain, and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain. EGF R has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, heparin-binding EGF, and neuregulin-2 alpha, in the absence of a co-receptor. Ligand binding induces EGF R homodimerization as well as heterdimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation, and cell signaling. EGF R can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGF R signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGF R signaling has also been shown to play a role in carcinogenesis.1-3\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested. \n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nStore at 4 degrees C before opening. DO NOT FREEZE. This product is stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Protect from light.

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SPECIFICATIONS

Catalog Number

E3375-01B1

Size

100Tests

Applications

FC

Hosts

Rat

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with APC.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Daly, R.J., (1999) Growth Factors, 16:255-263. 2. Schlessinger, J., (2000) Cell. 103:211-225. 3. Maihle, N.J. et al., (2002) Cancer Treat. Res. 107:247-258.

Additional Info

Recognizes human EGFR.

Alternative Names

EC=2.7.10.1, ERBB1, MENA