Epidermal Growth Factor Receptor (erbB, EGFR)

Cat no: E3375-01B

Supplier: United States Biological

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Additional Information

Epidermal Growth Factor Receptor (EGFR), also named erythroblastic leukemia viral oncogene homolog 1 (ErbB1), is a member of the type I receptor tyrosine kinase superfamily that also includes ErbB2-4. Applications: Suitable for use in Flow Cytometry and Direct ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry: 25ug/ml. Use 10ul to label 1-2.5x10e5 A431 cells in a total reaction volume < 200ul. Direct ELISA: 0.5-1ug/ml. The detection limit is ~5ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.�

Catalogue number:	E3375-01B
Reactivities:	Human
Hosts:	Rat
Applications:	ELISA, Flow Cytometry
Size:	100ug
Form:	Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul PBS.
P type:	Mab
Isotype:	IgG2a
Purity:	Purified by Protein G affinity chromatography.
Alternative names:	EC=2.7.10.1, ERBB1, MENA
Additional info:	Recognizes human Epidermal Growth Factor Receptor. Does not crossreact with recombinant human ErbB2, ErbB3 or ErbB4.

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Epidermal Growth Factor Receptor (erbB, EGFR)

Cat no: E3375-01B

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