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Epidermal Growth Factor Receptor (erbB, EGFR)

Cat no: E3375-01D

Epidermal Growth Factor Receptor (erbB, EGFR)

The EGF receptor (EGFR), a 180kD transmembrane tyrosine kinase, is part of the ErbB family which mediate ligand induced signaling as heterodimers. Ligand binding results in activation of the intrinsic kinase activity and autophosphorylation on tyrosine residues within and surrounding the kinase domain. The phospho-tyrosine residues serve as docking sites for adapter proteins that nucleate signaling complexes that result in the activation of the Ras, PI-3 kinase and PLC-g pathways. EGFR, which transduces the biological effects of EGF and TGF-a, is frequently over-expressed in solid tumors. \n\nApplications: \nSuitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:10,000. Lysate from A431 cells was resolved by electrophoresis, transferred to Immobilon-P and probed with E3375-01D (1:10,000). Proteins were visualized using IgG Pab Gt x Rb (HRP) secondary antibody and ECL detection. \nImmunoprecipitation: 1:30 using Protein G agarose beads.\nFlow Cytometry: 1:10. Permeablization of cells required\nImmunofluorescence: 1:50, paraformaldehyde fixed cells.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nA431 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3375-01D

Size

100ul

Applications

FC, IF, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 30mM Tris-Glycine, pH 7.4, 90mM sodium chloride, 0.03% BSA, 0.006% sodium azide, 40% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

1. Buchanan, F Gregory, et al (2003) J Biol Chem 278:35451-7. 2. Moghal, N and Neel, B G (1998) Mol Cell Biol 18:6666-78.

Additional Info

Recognizes the C-terminal domain of human EGFR at ~170kD. Does not crossreact with any other ErbB family member. Does not recognize mouse or rat.

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