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Epidermal Growth Factor Receptor (erbB, EGFR)

Cat no: E3375-04B

Epidermal Growth Factor Receptor (erbB, EGFR)

Epidermal growth factor receptor (EGFR) is a 170kDa transmembrane glycoprotein receptor tyrosine kinase that, when activated by epidermal growth factor EGF), affects cell growth and differentiation in normal and cancer cells (1). Binding of EGF or TGF alpha to EGFR \nactivates tyrosine kinase activity of the receptor (2). The carboxy terminal tyrosine residues on EGFR, Tyr 1068, Tyr 1148, and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding 3). EGFR overexpression is exhibited various cancer, such \nas glioma, colorectal carcinoma, breast carcinoma, and head and neck carcinoma (4). The phosphorylation level of EGFR is considered one of the important predicators for outcome in \nnon-small cell lung cancer therapy (5). \n \nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunocytochemistry. Other applications not tested.\n \nRecommended Dilutions: \nWestern Blot: 1:1000-5000 \nImmunocytochemistry: 1: 100-250 \nFlow Cytometry: 1:55 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

E3375-04B

Size

100ul

Applications

FC, ICC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Hunter T, et al. Nature 311: 414

Additional Info

Recognizes both phosphorylated and non-phosphorylated EGFR protein.

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