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Epidermal Growth Factor Receptor (erbB, EGFR, EC 2.7.10.1, ERBB, ERBB1, HER1, MENA, PIG61)

Epidermal Growth Factor Receptor (erbB, EGFR, EC 2.7.10.1, ERBB, ERBB1, HER1, MENA, PIG61)

Cat no: E3375-03D

Supplier: United States Biological
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Mutations to EGFR have been found in many human carcinomas including breast, brain, lung, and colon. Over-expression of more than one member of the ErbB family was found to have a negative synergistic effect on patient outcome. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:8000-1:32,000 Western Blot: 1:500-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E3375-03D
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: ELISA, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: Avian Erythroblastic Leukemia Viral (v-erb-b) Oncogene Homolog, Wa2, Wa5
References: 1. Sebastian, S. et al. (2006) The complexity of targeting EGFR signalling in cancer: from expression to turnover. Biochim. Biophys. Acta. 1766: 120-39. 2. Normanno, N. et al. (2006) Epidermal growth factor receptor (EGFR) signaling in cancer. Gene. 366: 2-16.
Additional info: Recognizes human EGF R. Species Crossreactivity: rat, mouse.

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