Epidermal Growth Factor Receptor (erbB, EGFR), Extracellular Domain (BSA & Azide Free)

Applications: Immunoprecipitation (Native only), Inhibition of Cell Proliferation, Blocks EGF/TGFa-Induced Activation of EGFR. \nRecommended Dilution:\nImmunoprecipitation (Native only): Use Protein G; Ab at 2microg/mg protein lysate)\nTo block EGF/TGFa-induced activation of EGFR: 0.5mg/ml, induce cells with 30ng/ml EGF\nOptimal dilutions to be determined by the researcher.

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SPECIFICATIONS

Catalog Number

E3375-04X

Size

100ug

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum, NHP

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.\nAlso available with BSA and azide. See E3375-04.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Kawamoto T, et. al., (1983) Proceedings of the National Academy of Sciences USA 80:1337-41. 2. Sato JD, et. al., (1983) Molecular Biology and Medicine 1(5):511-29. 3. Wikstrand CJ, et. al., (1995) Cancer Research 55(14):3140-8

Additional Info

Recognizes the 170kD (intact) and 145kD (VIII variant) forms of EGF receptor. The epitope maps to the extracellular domain of EGFR. Species Reactivity: Human, Chimpanzee and Monkey. Does not react with Mouse or Rat.