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Epidermal Growth Factor Receptor (erbB, EGFR),    phosphorylated (Tyr845)

Cat no: E3375-04G

Epidermal Growth Factor Receptor (erbB, EGFR), phosphorylated (Tyr845)

Epidermal growth factor receptor (EGFR) is a 175kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the \nreceptor (2). The epidermal growth factor (EGF) receptor is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C Ca2+/phospholipid-dependent enzyme) mediated phosphorylation at identified sites. Analysis of predicted secondary structure of the EGF receptor reveals that all of the phosphorylation sites are located near beta turns (3). I t has been reported that stimulating human glioblastoma cells with recombinant HGF induces biologically relevant EGFR activation. EGFR phosphorylation at Tyr(845) and Tyr(1068) increased 6 to 24 h after cell stimulation with HGF and temporally coincided with the induction of transforming growth factor-alpha (~5-fold) and HB-E, GF (~23-fold) expression, Tyr(845) and Tyr(1068) phosphorylation, in response to HGF, \nwas inhibited by cycloheximide and actinomycin D, consistent with a requirement for DNA transcription and RNA translation 4). \n \nApplications: \nSuitable for use in Immunocytochemistry, Western Blot. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1:500 \nImmunocytochemistry: 1:100-250 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

E3375-04G

Size

100ul

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Hunter T. et al. Nature 311: 414

Additional Info

Recognizes EGFR phosphorylated on tyrosine 845.

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