Epidermal Growth Factor Receptor (erbB, EGFR), phosphorylated (Tyr992)

Epidermal growth factor (EGF) receptor is a 170kD single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation (1,2). Tyrosine 845 resides in the activation loop of the receptor kinase domain (3,4). Phosphorylation of Tyr845 may stabilize the activation loop, maintain the enzyme in an active state and provide a binding surface for substrate proteins. It has been shown that c-Src is involved in phosphorylation of the EGF receptor on Tyr845, which is associated with modulation of receptor function (5). Phospho-tyrosine 992 of activated EGF receptor is a direct binding site for the phospholipase C-gamma (PLCgamma) SH2 domain (6). This binding results in activation of PLC-gamma-mediated downstream signaling. Phosphorylation of Tyr1045 creates a major docking site for c-Cbl (7). Binding of c-Cbl to the activated EGF receptor leads to assembly of ubiquitination machinery to the receptor, and enables receptor ubiquitination and degradation (8). Phospho-tyrosine 1068 of activated EGF receptor is a direct binding site for the Grb2/SH2 domain (9). This binding results in Ras activation through a Grb2/Sos-1 signaling mechanism (2). \n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot: 1:1000 \nImmunohistochemistry (Paraffin): 1:100.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3375-53C

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Hackel, P.O. et al. (1999) Curr. Opin. Cell Biol. 11, 184-189. 2. Zwick , E. et al. (1999) Trends Pharmacol. Sci. 20, 408-412. 3. Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-1054. 4. Hubbard, S.R. et al. (1994) Nature 372, 746-754. 5. Biscardi, J.S. et al. (1999) J. Biol. Chem. 274, 8335-8343. 6. Emlet, D.R. et al. (1997) J. Biol. Chem. 272, 4079-4086. 7. Levkowitz, G. et al. (1999) Mol. Cell 4, 1029-1040. 8. Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-1866. 9.. Rojas, M. et al. (1996) J. Biol. Chem. 271, 27456-27461. 10. Iordanov, M. S. et al. (2002) Mol. Cell. Biol. 22, 5380-5394.

Additional Info

Recognizes human overexpressed EGF receptor only when phosphorylated at Tyr992 at 175kD. Does not crossreact with other phosphorylated receptor tyrosine kinases. Species sequence homology: Mouse and rat-100%.