Epidermal Growth Factor Receptor, phosphorylared (Ser695) (EGFR, ErbB, ErbB 1, ErbB1, ErbB-1, mENA, Oncogene ErbB, Receptor Tyrosine Protein Kinase ErbB1, Wa2, Wa5)

Epidermal growth factor receptor (EGFR) is a 175kD transmembrane glycoprotein receptor tyrosine kinase that is involved in diverse cellular functions including cell proliferation, differentiation, motility, survival and in tissue development (1, 2). EGFR is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C (Ca2+/ phospholipid-dependent enzyme) mediated phosphorylation at identified sites. EGFR contains additional phosphorylation sites including a prominent phosphothreonine and several phosphoserines which account for the majority of phosphate covalently bound to the receptor in vivo. Analysis of predicted secondary structure of EGFR reveals that all of the phosphorylation sites are located near beta turns (3).\n\nApplications:\nSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000\nImmunocytochemistry: 1:100-1:250 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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