Epidermal Growth Factor Receptor, phosphorylated (Ser1047) (erbB, EGFR)

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. The EGF Receptor (EFR) is activated upon stimulation with growth factors of the EGF family and autophosphorylates at multiple tyrosine residues. Serine phosphorylation of the carboxy terminal domain at Serine 1047 leads to ligand-dependent EGFR desensitization. However, if serine 1047 is phosphorylated, the neighboring tyrosine 1045 may not be phosphorylated due to sterical hindrance, thus inhibiting cbl-mediated receptor degradation. E3375-53D specifically recognizes EGFR phosphorylated at Serine 1047. Applications: Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 0.1 ug/ml Western Blot: 0.5ug/ml for HRP/ECL detection. Immunocytochemistry: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Cell lysate of 2 x 10e6 vanadate treated HepG2 cells. Supplied as a lyophilized powder. Reconstitute with 200ul ddH2O. After complete solubilization of the proteins, add 200ul SDS-PAGE sample buffer and incubate at 90 degrees C for 5 minutes. Western Blot : 20ul/lane HRP/ECL Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.