The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
Upon activation of PKC, the EGF Receptor is phosphorylated at Threonine 654. The phosphorylation of activated EGFR at Threonine 654 inhibits ubiquitination and degradation of the EGFR and thus diverts internalized EGFR from the degradative pathway to the recycling endosome.
Applications:
Suitable for use in ELISA and Western Blot.. Other applications not tested.
Recommended Dilution:
ELISA: 0.05ug/ml
Western Blot: 0.5ug/ml for HRP/ECL detection.
Optimal dilutions to be determined by the researcher.
Positive Control:
Lysate from PMA/pervanadate treated HepG2 cells. Supplied as a lyophilized powder. Reconstitute with 200ul ddH2O. After complete solubilization of the proteins, add 200ul SDS-PAGE sample buffer and incubate at 90 degrees C for 5 minutes.
Western Blot : 20ul/lane HRP/ECL
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Quick Search
Products 
