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Epidermal Growth Factor Receptor, phosphorylated (Thr693) (erbB, EGFR)

Cat no: E3375-04E

Epidermal Growth Factor Receptor, phosphorylated (Thr693) (erbB, EGFR)

Epidermal growth factor receptor (EGFR) is a 175kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the \nreceptor (2). The epidermal growth factor (EGF) receptor is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C (Ca2+/ \nphospholipid- dependent enzyme) mediated phosphorylation at identified sites. The EGF receptor contains additional phosphorylation sites including a prominent phosphothreonine \nand several phosphoserines which account for the majority of phosphate covalently bound to the receptor in vivo. Analysis of predicted secondary structure of the EGF receptor reveals \nthat all of the phosphorylation sites are located near beta turns (3). \n \nApplications: \nSuitable for use in Western Blot Immunoprecipitation, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1:10,000-20,000 \nImmunocytochemistry: 1:100-250 \nImmunoprecipitation: 1:40 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

E3375-04E

Size

100ul

Applications

ICC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Hunter T, et al. Nature 311: 414

Additional Info

Recognizes human EGFR phosphorylated on Thr693. \n

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