Epidermal growth factor receptor (EGFR) is a 175kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the
receptor (2). The epidermal growth factor (EGF) receptor is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C (Ca2+/
phospholipid- dependent enzyme) mediated phosphorylation at identified sites. The EGF receptor contains additional phosphorylation sites including a prominent phosphothreonine
and several phosphoserines which account for the majority of phosphate covalently bound to the receptor in vivo. Analysis of predicted secondary structure of the EGF receptor reveals
that all of the phosphorylation sites are located near beta turns (3).
Applications:
Suitable for use in Western Blot Immunoprecipitation, Immunocytochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot: 1:10,000-20,000
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:40
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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