Epidermal Growth Factor Receptor, phosphorylated (Tyr1068) Blocking Peptide (erbB, EGFR),

Epidermal growth factor (EGF) receptor is a 170kD single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation (1,2). Tyrosine 845 resides in the activation loop of the receptor kinase domain (3,4). Phosphorylation of Tyr845 may stabilize the activation loop, maintain the enzyme in an active state and provide a binding surface for substrate proteins. It has been shown that c-Src is involved in phosphorylation of the EGF receptor on Tyr845, which is associated with modulation of receptor function (5). Phospho-tyrosine 992 of activated EGF receptor is a direct binding site for the phospholipase C-gamma (PLC-gamma) SH2 domain (6). This binding results in activation of PLC-gamma-mediated downstream signaling. Phosphorylation of Tyr1045 creates a major docking site for c-Cbl (7). Binding of c-Cbl to the activated EGF receptor leads to assembly of ubiquitination machinery to the receptor, and enables receptor ubiquitination and degradation (8). Phospho-tyrosine 1068 of activated EGF receptor is a direct binding site for the Grb2/SH2 domain (9). This binding results in Ras activation through a Grb2/Sos-1 signaling mechanism (2)\n\nProduced by immunizing rabbits with a synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr1068 of human EGF receptor.\n\nCorresponding antibody, E3375-53A, detects endogenous levels of EGF receptor only when phosphorylated at Tyr1068. Does not recognize other sites of tyrosine phosphorylation on EGF receptor, nor does it crossreact with other tyrosine-phosphorylated proteins. Species Crossreactivity: Mouse and rat.\n\nApplications: \nSuitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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