Epidermal Growth Factor Receptor, phosphorylated (Tyr1173) (erbB, EGFR)

Epidermal Growth Factor Receptor, also known as ErbB-1 (EGFR, a 185kD glycoprotein) is a transmembrane tyrosine kinase that regulates a variety of biological responses ranging from mitogenesis to stress signaling. The EGFR consists of a large extracellular domain, a single transmembrane domain and a cytoplasmic domain that exhibits kinase activity. Upon binding of EGF to the extracellular domain, the receptor undergoes dimerization and becomes phosphorylated on several tyrosine residues within the cytoplasmic domain. Autophosphorylation of tyrosine 1173 creates a major binding site for the protein tyrosine phosphatase SHP-1, which can dephosphorylate the EGFR and thereby block EGFR-induced activation of the ERK1&2 signaling pathway.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1ug/ml for AP/BCIP/NBT (MTT) detection; 0.1ug/ml for HRPO/ECL deterction; Use 0.5% Caseine, 0.5% BSA in PBST for blocking.\nImmunoprecipitation: 1-10ug per 10e6 vanadate treated A431 cells.\nELISA: 0.05ug/ml\nOptimal dilution to be determined by researcher.\n\nPositive Control:\nH2030-03H1-Cell lysate from vanadate-treated HepG2 cells.\nAdd 100ul SDS-PAGE sample buffer and incubate at 90 degrees C for 5 minutes. Aliquot and store at -20 degrees C \nWestern Blot: 20ul/lane (mini gel) for HRP/ECL detection.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3375-15E

Size

50ug

Applications

ELISA, ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in PBS, 0.09% sodium azide, PEG and sucrose before the addition of glyceol to 40%

P Type

Mab

Purity

Purified by subsequent thiophilic absorption and size exclusion chromatography.

Isotype

IgG1

Additional Info

Recognizes the N1170AEpYLRV motif corresponding to the major autophosphorylation site of human EGF receptor. Does not cross-react with the non-phosphorylated human, mouse, rat and dog EGF receptor or with unrelated Tyr-phosphorylated proteins. Species Crossreactivity: mouse.