Epidermal Growth Factor Receptor, phosphorylated (Tyr1173) (erbB, EGFR)

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.\n\nBinding of EGF to the extracellular domain of the EGF-receptor results in receptor dimerization and autophosphorylation on tyrosine residues, Y1173 being the major autophosphorylation site. E3375-55B specifically interacts with the 1170-NAEpYLRV motif corresponding to the major autophosphorylation site of human EGFR. The antibody does not interact with the non-phosphorylated EGFR nor with unrelated Tyrosine-phosphorylated proteins. This phosphorylation site-specific monoclonal antibody allows the detection and quantification of activated EGFR in crude cell extracts in Immunoblot, ELISA and Biosensor assay formats without the use of P32.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.05ug/ml\nWestern Blot: 0.1ug/ml for HRP/ECL detection.\nImmunoprecipitation: 1-10ug per 106 vanadate treated A431 cells.\nImmunocytochemistry: 1-10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nLysate from PMA/pervanadate treated HepG2 cells. Supplied as a lyophilized powder. Reconstitute with 200ul ddH2O. After complete solubilization of the proteins, add 200ul SDS-PAGE sample buffer and incubate at 90 degrees C for 5 minutes.\nWestern Blot : 20ul/lane HRP/ECL\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3375-55B

Size

100ug

Applications

ELISA, ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Can

Form

Supplied as a lyophilized powder from PBS, PEG, sucrose, 0.09% sodium azide. Reconstitute with 1ml water for 15 minutes at room temperature.

P Type

Mab

Purity

Purified by thiophilic adsorption and size exclusion chromatography.

Isotype

IgG1,k

Additional Info

Recognizes the 1170-NAEpYLRV motif corresponding to the major autophosphorylation site of human EGFR. Does not interact with the non-phosphorylated EGFR nor with unrelated Tyrosine-phosphorylated proteins. Species crossreactivity: mouse, canine.