EPS15L1 (epidermal growth factor receptor substrate 15-like 1), also known as EPS15R (EPS15-related), is structurally related to the EPS15 protein, a protein involved in the receptor-mediated endocytosis of the EGF receptor. EPS15L1 and EPS15 both contain an EH (Eps15 homology) domain, a protein-protein interaction domain found in several proteins that participate in endocytosis, vesicle transport, and signal transduction.
Uses:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Western Blot: 1:2000-1:10000
immunoprecipitation: 2-5ug/mg lysate
Optimal dilution determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.