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Epidermal Growth Factor Receptor, T790M, L858R, Recombinant, Human, GST-Tag

Cat no: E3375-47E

Epidermal Growth Factor Receptor, T790M, L858R, Recombinant, Human, GST-Tag

The L858R mutation has been associated with non-small cell lung cancer patients who demonstrate clinical responsiveness to the tyrosine kinase inhibitor gefitinib (Iressa, ZD1839).\n\nSource: \nRecombinant human EGFR T790M L858R, aa668-1210, with N-terminal GST tag, expressed in baculovirus expression system. \n\nMolecular Weight: 88kD\n\nSpecific Activity: \n667pmol/min/ug\n\nReaction Buffer: \n50mM HEPES (pH7.5), 10mM MgCl2, 1mM EDTA, 0.01% BRIJ- 35 and 200uM of ATP.\n\nSubstrate: \n4uM Tyr peptide 4 from Invitrogen.\n\nApplications: \nUseful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution: \nThe enzyme reaction was carried out for 1h at room temperature in reaction buffer. Optimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

E3375-47E

Size

10ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 138mM NaCl, 0.05% Tween-20, 50% glycerol, 3mM DTT.

Purity

(same/more than)80%

References

1. Adachi,S. et al., Cancer Res. 67 (13), 6493- 6501 (2007). 2. Rosano,L. et al., Cancer Res. 67 (13), 6351- 6359 (2007).

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