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Epidermal Growth Factor, Recombinant, Human (E. coli) (EGF)

Cat no: E3410

Epidermal Growth Factor, Recombinant, Human (E. coli) (EGF)

Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human EGF is a 6.2kD protein containing 53 amino acid residues.\n\nAA Sequence:\nNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR\n\nBiological Activity:\nED50 calculated by the dose-dependent proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is <0.1ng/ml corresponding to a specific activity of 1x10e7units/mg.\n\nProtein Content:\nEGF quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).\n2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard.\n\nStorage and Stability: \nLyophilized powder may be stored at -20 degrees C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3410

Size

100ug

Form

Supplied as a lyophillized powder. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to (same/more than) 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

(same/more than) 98% ([SDS-PAGE and HPLC)

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