Epidermal Growth Factor, Recombinant, Rat (EGF, Pro-epidermal Growth Factor)

Supplier:

United States Biological

Cat no: E3374-11U

Epidermal Growth Factor, Recombinant, Rat (EGF, Pro-epidermal Growth Factor)

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53aa peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Epidermal Growth Factor Rat Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 53aa and having a molecular mass of 6151D. The Rat EGF is purified by proprietary chromatographic techniques.\n\nSource:\nRecombinant corresponding to rat EGF expressed in E. coli.\n\nAA Sequence: \nNSNTGCPPSYDGYCLNGGVCMYVESVDRYVCNCVIGYIGERCQHRDLRWWKLR\n\nMolecular Weight: \n~6.151kD\n\nBiological Activity: \nThe ED50 as calculated by the dose-dependent proliferation of murine BALB/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of >1x10e7units/mg.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3374-11U

Size

20ug

Form

Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with sterile ddH2O to (same/more than)100ug/ml.

Purity

~98% (SDS-PAGE, HPLC)

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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