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Epigen, Recombinant, Human

Cat no: E3376-25

Epigen, Recombinant, Human

Epigen is an EGF-related polypeptide growth factor that signals through the ErbB receptor-1. It is produced in several tissues, including the testis, liver, heart and in certain tumor cells. Epigen is mitogenic for fibroblasts and epithelial cells. Human Epigen is initially synthesized as a glycosylated 14.7kD transmembrane precursor protein, which is processed by proteolytic cleavage to produce a mature soluble sequence. Recombinant human Epigen is a 7.9kD monomeric protein, containing 72 amino acid residues, which comprises the EGF homologous portion of the Epigen precursor.\n\nSource:\nE. coli\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile buffer, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nBiological Activity:\nDetermined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells. The expected ED50 for this effect is 150-300ng/ml.\n\nCountry of Origin:\nUSA

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SPECIFICATIONS

Catalog Number

E3376-25

Size

5ug

Form

Supplied as a lyophilized powder.

Purity

(same/more than) 98% by SDS-PAGE and HPLC analyses.

Read more on Supplier website

SUPPLIER INFO

United States Biological

Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, IHC, WB

Hosts

Mouse

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Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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