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Epigen, Recombinant, Human

Cat no: E3376-25B

Epigen, Recombinant, Human

Epigen was identified as an EGF family member by amino acid sequence homology (1). The sequence was obtained from mouse keratinocyte mRNA. Epigen is expressed in normal human mammary tissue and in some prostate and breast carcinomas (2). Epigen binding to known EGF receptors is with lower affinity when compared to EGF. However, Epigen appears to be superior in inducing cell proliferation (2). Epigen induces responses in MDA-MB-468 breast carcinoma cells. Epigen also has effects on prostate cells and vascular endothelial cells (2). Epigen binds to ErbB1/Her1 (2). Binding causes receptor homodimerization and heterodimerization with other Erb/Her receptors (2). \n\nBioactivity:\nDetermined in a MCF-10A cell proliferation assay. The ED50 of each lot is between 2-30ng/ml.\n\nEndotoxin\n(same/less than) 0.01ng endotoxin/1ug hEpigen.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions o be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile buffer. Aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3376-25B

Size

10ug

Applications

WB

Reactivities

Hum

Form

Supplied as lyophilized powder from 20mM citrate, pH 3, 100mM NaCl and 20ug BSA per 1ug hEpigen.

Purity

(same/more than) 98% by SDS-PAGE

References

1. Strachan, L. et al. (2001) J Biol Chem 276, 18265-71. 2. Kochupurakkal, B.S. et al. (2005) J Biol Chem 280, 8503-12.

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SUPPLIER INFO

United States Biological

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

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Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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