Epiregulin, Recombinant, Human, Val63-Leu108 (EREG)

Epiregulin is a member of the EGF family that is highly expressed in many cancer-specific cells, cells of the placenta and macrophages (1,2). Epiregulin induces keratinocyte and corneal epithelial cell proliferation, oocyte maturation and may function in blastocyst implantation during pregnancy (3-5). Active soluble epiregulin is produced by proteolytic processing of a transmembrane precursor. Epiregulin is the only EGF family member known to bind to both the ErbB1/HER1 and ErbB4/HER4 EGF receptors (6). Epiregulin binding induces receptor homodimerization or heterodimerization with other Erb/Her receptors. Epiregulin activates the ERK1/2, and PI3K/Akt pathways (1,3). \n\nSource: \nProduced in E. coli.\n\nMolecular Characterization:\nBased on amino acid sequencing, greater than 50% of recombinant hEpiregulin has a Met on the amino-terminal Val63 (MVSIT) and has a calculated MW of 5,406. The remainder starts at Val63 (VSITK). DTT-reduced and non-reduced protein migrate as 5 kDa polypeptides. \n\nEndotoxin:\n(same/less than) 0.01ng endotoxin/1ug hEpiregulin. \n\nBioactivity:\nThe bioactivity of recombinant hEpiregulin was determined in a MCF 10A cell proliferation assay. The ED 50 of each lot is between 0.10-0.85 ng/ml.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nStable in lyophilized state at 4 degrees C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4 degrees C for 2 months and at -20 degrees C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20 degrees C should be in a manual defrost \nfreezer.

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SPECIFICATIONS

Catalog Number

E3376-75C

Size

10ug

Applications

WB

Reactivities

Hum

Form

Supplied as lyophilized powder from a 0.22um filtered solution of 20mM citrate, pH 3.0 containing 100mM NaCl and 20ug BSA per 1ug hEpiregulin. Reconstitute with sterile 20mM citrate, pH 3.0 to a final hEpiregulin concentration of greater than 50ug/ml. Solubilize for 30 \nminutes at RT with occasional gentle vortexing.

Purity

(same/more than)98% as determined by SDS-PAGE of 6ug reduced (+) and non-reduced (-) recombinant hEpiregulin.

References

1. Toyoda, H. et al. (1997) Biochem J 326 ( Pt 1), 69-75, 2. Re?villion, F. et al. (2008) Ann Oncol 19, 73-80, 3. Morita, S. et al. (2007) Mol Vis 13, 2119-28, 4. Shirakata, Y. et al. (2000) J Biol Chem 275, 5748-53, 5. Song, H. et al. (2000) Mol Endocrinol 14, 1147-61, 6. Riese, D.J. et al. (1998) J Biol Chem 273, 11288-94.