Episialin

This product is a transmembrane glycoprotein with a large mucin-like extracellular domain that matures through several intermediate forms generated by proteolysis, and sequential addition and processing of numerous O-linked glycans that are heavily sialylated. The gene encoding episialin (MUC1) is highly polymorphic and each allele encodes a product that contains a different number of repeats (between 30 and 90) leading to large differences in molecular weight of the protein.\nEpisialin is present in several secretory epithelial cell types.\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:25-1:200\nImmunohistochemistry: 1:25-1:200, use ABC as detection reagent.\nWestern Blot: 1:100-1:1000\nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nDerived by fusion of mouse myeloma cells with spleen cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3413-01

Size

100ul

Applications

FC, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Gourevitch, M. M., et al., (1995) Br J Cancer 72:934-938. 2. Norum, L. F., et al., (1998) Tumour Biol 19:134-146. 3. Norum, L. F., et al., (2001) Tumour Biol 22:216-22.

Additional Info

Recognizes human Episialin. Reacts with all glycoforms of Episialin.

Alternative Names

MUC1, MAM-6, CA 15-3, PEM and EMA.