Epithelial Sodium Channel, gamma (ENAC Gamma)

Tissue acidosis (decrease in pH below the physiological level) that occurs in ischemia, tissue damage or inflammation is accompanied by pain. At the molecular level, H+-gated cation channels are activated by low pH in nociceptive neurons. H+-gated cation channels, members of the NaC/DEG superfamily of Na channels that include amiloride-sensitive epithelial Na+ channel proteins (alpha, beta, and gamma, and delta-ENaC subunits), are expressed in epithelia of the vertebrate kidney, colon, lucolon and other tissues. ENaC-b from various species are ~80% identical, and only ~30% similarity with the ENaC-alpha ENaC-delta (human 638 aa) is expressed mainly in brain, pancreas, testis, and ovary. It is 27-30% homologous with the other ENaCs. It can associate with b, and g ENaC to form a functional channel.\n\nApplications: \nSuitable for use in ELISA, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000\nELISA: 1:10,000-50,000, control peptide can be used to coat plates at 1ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3414-75A

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Canessa CM et al (1993) Nature 361:463-467. 2. Linguela E et al (1994) J. Biol. Chem. 269:13736-13739. 3. Kreutz R et al (1997) Hypetension 29:131-136. 4. Voilley N et al (1995) Genomics 28:560-565. 5. McDonald FJ et al (1994) Am. J. Physiol. 268:1157-1163. 6. Thomas CP et al (1996) 271:26062-26066. 7. Garty H and Palmer LG (1997) Physiol. Reviews. 77:359-396 (review). 8. Cristia E, 2007, The Jornal of Physiology, volume 578, Issue 2: 413-424., IHC. 9. Sch;mitt, R., 2003, Am j Physiol renal Physiol, 284: 1097-1104, WB, IHC.

Additional Info

Recognizes rat ENAC gamma. Species Sequence Homology: mouse (100%), human (92%), rabbit (80%). No significant homology is detected with ENAC alpha, beta, delta subunits.