Epithelial Sodium Channel, gamma (ENAC Gamma)

Tissue acidosis (decrease in pH below the physiological level) that occurs in ischemia, tissue damage or inflammation is accompanied by pain. At the molecular level, H+-gated cation channels are activated by low pH in nociceptive neurons. H+-gated cation channels, members of the NaC/DEG superfamily of Na channels that include amiloride-sensitive epithelial Na+ channel proteins (alpha, beta, and gamma, and delta-ENaC subunits), are expressed in epithelia of the vertebrate kidney, colon, lung, tongue, and brain. The ENaC subunits may form heterotrimeric active Na channel. ENaCs are involved in Na and water readsorption, and salty taste transduction) of vertebrate colon, lung, kidney and tongue. The superfamily of DEG/NaC proteins are characterized by intracellular N and C-termini, two TM domains, and a large extracellular loop. ENaC-beta subunit (mouse/rat 638 aa, human 640 aa), ENaC-gamma subunit (mouse 655 aa, rat 650 aa, and human 649 aa) are expressed in lung, kidney, colon and other tissues. ENaC-b from various species are ~80% identical, and only ~30% similarity with the ENaC-alpha ENaC-delta (human 638 aa) is expressed mainly in brain, pancreas, testis, and ovary. It is 27-30% homologous with the other ENaCs. It can associate with b, and g ENaC to form a functional channel.\n\nApplications: \nSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml\nELISA: 0.5-1ug/ml.\nImmunohistochemistry: 2-20ug/ml. See references.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: E3414-75H Epithelial Sodium Channel, gamma, Rat Control Peptide \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

E3414-75D

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as liquid in PBS, pH 7.4 and 0.1% BSA, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Canessa CM et al (1993) Nature 361:463-467. 2. Linguela E et al (1994) J. Biol. Chem. 269:13736-13739. 3. Kreutz R et al (1997) Hypetension 29:131-136. 4. Voilley N et al (1995) Genomics 28:560-565. 5. McDonald FJ et al (1994) Am. J. Physiol. 268:1157-1163. 6. Thomas CP et al (1996) 271:26062-26066. 7. Garty H and Palmer LG (1997) Physiol. Reviews. 77:359-396 (review).

Additional Info

Recognizes rat Epithelial Sodium Channel, gamma (ENAC Gamma). Species sequence homology: murine 100%, human 92%, rabbit 80%.