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Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1)

Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1)

Cat no: E3414-05H

Supplier: United States Biological
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Cellular Localization: Cell Membrane Positive Control: Breast Carcinoma and human lung Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 1:50-1:100 using ABC method. Enzymatic predigestion of paraffin embedded tissue is recommended prior to immunostaining. Western Blot: 1:100-1:750 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E3414-05H
Reactivities: Human
Hosts: Mouse
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 500ul
Form: Supplied as a liquid in 20 mM Tris-borate, 150 mM sodium chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified
Alternative names: EpCam
References: 1. Winter MJ et al. (2003) Am J Pathol 163:2139-48.
Additional info: This antibody reacts with two glycoproteins of 34 and 49kD present on the epithelial cells. It stains with simple epithelia and basal layers of stratified non-keratinized squamous epithelium. Species Crossreactivity: Crossreacts with Human.

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