Epithelial antigen is a membrane bound, glycosylated phosphoprotein anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It may play a role in adhesive functions and in cell to cell interactions, metastasis and signalling and provide a protective layer on epithelial surfaces. It is capable of direct or indirect interaction with actin cytoskeleton. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:100-1:750
Immunohistochemistry (formalin fixed paraffin embedded): 1:50-1:100
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
Positive Control:
Human lung cell line
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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