Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1, MOC31, Ber-EP4)

This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which have been described as tumor-specific molecules on carcinomas. Ep-CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, and de novo expression being seen in dysplastic squamous epithelium.\n\nRecommended Positive Control Tissue: Human colon.\n\nApplications:\nImmunohistochemistry: (frozen)- Neat, (paraffin)- Neat\n5ml/50 Tests.\nOptimal working dilutions to be determined by researcher.\n\nImmunohistochemistry: Requires protein digestion pre-treatment of paraffin sections e.g. 0.1% pronase, 10 minutes.\n\nRecommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP \n\nFusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of a mouse myeloma cell line.\n\n

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SPECIFICATIONS

Catalog Number

E3415-05A

Size

5ml

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG1

References

1. Edwards, P.A.W. (1985) Br. J. Cancer 51:149.

Additional Info

Recognizes a surface glycoprotein of approximately 35kD.The monoclonal antibody reacts with all human epithelial cell types except hepatocytes, gastric parietal cells, superficial layers in squamous stratified epithelia, sebaceous glands, cortical thymic epithelium, ovarian follicular epithelium, ependyma and plexus epithelium. Recognizes all carcinoma types tested so far.