EPOR (Erythropoietin receptor, Epo-R)

Erythropoietin receptor (Epo-R) belongs to the cytokine receptor family and is a 507 amino acid type I transmembrane protein. EpoR pre-exists as dimers which changes the homodimerized state after binding of its 34 kDa ligand erythropoietin (Epo) (1,2). Erythropoietin is the primary regulator of erythropoiesis, and promotes the survival, proliferation, and differentiation of erythroid progenitor cells. Both, Epo and Epo-R are essential for the production of red blood cells due to Epo exerts its function through the Epo receptor (3). The Epo-R is also expressed in many organs outside the bone marrow, suggesting that Epo is a pleiotropic anti-apoptotic factor. Signaling pathways have been shown to influence numerous cellular functions in normal and tumor cells, including proliferation, apoptosis, and drug resistance (4). \n\nApplications: \nSuitable for use in Flow Cytometry, ELISA and ELISA. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1.2ug/10e6 cells\nELISA: 1:200-1:400\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167732

Size

100ug

Applications

ELISA, FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Winkelmann JC (1992). The human erythropoietin receptor. Int J Cell Cloning. Sep;10(5):254- 61. 2. Livnah O, Stura EA, Middleton SA, Johnson DL, Jolliffe LK, Wilson IA (1999). Crystallographic evidence for preformed dimers of erythropoietin receptor before ligand activation. Science 283 (5404): 987

Additional Info

Recognizes human EPOR. Recognizes the complete native protein expressed on transfected mammalian cells.