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Epstein Barr Virus Early Antigen, IgG (EBV EA) BioAssay(TM) ELISA Kit

Cat no: E3440-03

Epstein Barr Virus Early Antigen, IgG (EBV EA) BioAssay(TM) ELISA Kit

The United States Biological Epstein Barr Virus Early Antigen (EBV-EA) IgG Enzyme-linked Immunosorbent Assay (ELISA), is intended for the qualitative detection of IgG antibody to Epstein Barr Virus Early Antigen in human serum and plasma.\n\nDetection Range: Qualitative Positive,; Negative control and Cut-off\nSample: 10ul\nSpecificity: 100%\nSensitivity: 100%\nTotal Time: 90min\n\nPrinciple of the Assay:\nPurified EBV-EA antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the anti- EBV-EA specific antibody, if present, will bind to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and TMB Chromogenic Substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgM specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls. \n \nKit Components:\nE3440-03A; Microwell Strips: EBV-EA antigen coated wells. (12 x 8 wells) \nE3440-03B: Sample Diluent. Blue color solution 1 vial (22ml) \nE3440-03C: Calibrator: Factor value (f) stated on label. Red cap. 1 vial (150ul)\nE3440-03D: Negative Control: Range stated on label. Natural Cap. 1 vial (150ul) \nE3440-03E: Positive Control: Range stated on label. Green Cap. 1 vial (150ul) \nE3440-03F: Washing Concentrate 10x: White Cap. 1 bottle (100ml) \nE3440-03G: Enzyme Conjugate: Red color solution. 1 vial (12ml) \nE3440-03H: TMB Chromogenic Substrate: Amber bottle. 1 vial (12ml)\nE3440-03I: Stop Solution: 2 N HCl. 1 vial (12ml) \n \nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E3440-03

Size

96Tests

Applications

ELISA

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