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Epstein Barr Virus VCA, IgG (EBA VCA) BioAssay(TM) ELISA Kit

Cat no: E3440-33

Epstein Barr Virus VCA, IgG (EBA VCA) BioAssay(TM) ELISA Kit

The United States Biological Epstein-Barr Virus-Viral Capsid Antigen (EBV-VCA) IgG Enzymelinked Immunosorbent Assay (ELISA), is intended for the detection and quantitative determination of IgG antibody to Epstein-Barr virus in human serum. A single serum specimen may be used to indicate previous infection or immune status with the Epstein-Barr virus.\n\nPurified EBV-VCA antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the EBV-VCA IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off, and TMB Chromogenic substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls. Detection of the Epstein-Barr virus was first described in 1964 by Epstein, Achong, and Barr using electron microscopic studies of cultured lymphoblasts derived from patients with Burkitt

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SPECIFICATIONS

Catalog Number

E3440-33

Size

96Tests

Applications

ELISA

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